Thin layer chromatogram (TLC) of an extract of thylakoid membranes from the leaf of annual meadow grass Poa annua. TLC plastic sheets are coated with a 60 F254 silica gel which measures 0. 2 millimetres thick. A drop of extract, corresponding to the column here, was laid at the bottom of the sheet. The sheet was then placed in a beaker of solvent (75% acetone & 25% petroleum ether). The picture shows the solubility of the extract in solvent. Six bands are seen; top (orange) is carotene; 2 (green) pheophytin; 3 (green) chlorophyll A; 4 (green) chlorophyll B; 5 (yellow) & 6 (mere trace) are carotenoids. The line across the top of image is the solvent line.