Tattoo needle puncture in skin and single eyebrow hair. Coloured scanning electron micrograph (SEM) of a piece of tissue from an eyebrow tattoo showing a single eyebrow hair surrounded by holes caused by the tattoo needle puncturing the skin. Skin tattooing has been practised for thousands of years. To make tattooing permanent tiny needles are used to punch through the top layer of the skin (epidermis) and into the next layer (dermis). Magnification: x48 when printed at 10cm wide